Phis-sumo vector

Author: mqfa

August undefined, 2024

WebbDownload scientific diagram Salient features of pET28a, design flaws and improved designs a Genetic elements present in pET28a include the φ10 (T7) promoter and the lac … Webb第1 页共2 页 Y187-pHis2YeastOne-HybridMediaKit使用说明书产品编号：YM1010（序号1-8），YM1011（YM1010+SL0930）储存温度：序号1-8常温保存；序号9需-20℃储存， …

Yeast two-hybrid vectors - Takara Bio

WebbWe have a modified pETM11 vector for the expression of SUMO3 fusion proteins available. This vector contains an optimized synthetic human sumo3 gene with appropiate … Webbsumo标签蛋白是一种小分子泛素相关修饰蛋白，是存在于真核生物中高度保守的参与蛋白质小泛素化相关修饰的一类大蛋白。与GST、MBP或NusA相比，SUMO不仅可以作为重组 … howley newfoundland canada

pHIS-parallel1 vector map and sequence

Webb27 feb. 2024 · However, removing SUMO is a labor- and time-consuming process. To enhance this system, here we show the construction of a bicistronic vector that allows … WebbpGADT7 AD is a yeast two-hybrid expression vector that is designed to express a fusion protein between the GAL4 activation domain (AD; amino acids 768–881) and a protein of interest. The fusion protein is expressed at high levels from the full-length ADH1 promoter. The fusion protein also contains a hemagglutinin (HA) epitope tag. WebbThe pSUMO vector is a low copy plasmid with kanamycin selection. ( b ) The DNA sequence encoding the SUMO tag. The restriction digestion site of enzyme BsaI is … howley park forterra

Phis-sumo vector

A M55V Polymorphism in a Novel SUMO Gene (SUMO-4) …

WebbFounded in 1996, LifeSensors, Inc. helps life scientists innovate in basic research, drug discovery and diagnostics. LifeSensors pioneered the SUMO-fusion system for high … WebbExamples of UBLs are SUMO, Rub1 (also called Nedd8), ISG15, Apg8 and Apg12. Once covalently attached to cellular targets, SUMO regulates protein:protein and protein:DNA …

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http://www.bioon.com.cn/doc/showarticle.asp?newsid=96627 WebbSUMO-4V constructs were made to express active recombinant proteins ending in the terminal di-glycine residues (Fig. 1, additional C-terminal residues are cleaved normally in …

Webbencoding amino acids 1-96 of SUMO-1 (lacking glycine 97 creating a SUMO-1 that is not susceptible to proteolytic removal by isopeptidases) was fused in frame to the amino … WebbIncludes: The Champion pET SUMO TA Cloning box contains linearized Champion pET SUMO vector, sterile water, dNTPs, 10X PCR Buffer, control template and primers, T4 DNA ligase, 10X ligation buffer, primers for sequencing or PCR screening, and an expression control. The SUMO Protease box contains SUMO Protease and buffers.

WebbpET SUMO (linearized) Linearized bacterial vector with 3'-T overhangs for TA cloning and inducible expression of an N-terminally 6xHis- and SUMO-tagged protein. Sequence … WebbWelcome to Vector Database! Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free …

WebbPhis2 1 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions …

Webbyeast 2-hybrid vectors (sundquist lab) pGADT7 and pGBKT7. Ligation Independent Cloning (LIC) vectors from Mark Connelly: pMCSG10, pMCSG7, pMCSG17-20, pMCSG19, … howley park sandstoneWebb11 dec. 2016 · pSUMO大肠杆菌表达载体说明 howley park leedsWebbpET-N-His-PreScission-SUMO是一种用于表达N端同时含有His标签 (His tag)和SUMO标签 (SUMO tag)双标签的目的蛋白的原核表达质粒。 His标签有利于通过亲和层析方法进行目 … howley park road east morley leeds ls27 0swWebb1. Centrifuge at 5,000×g for 5 min. 2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g. 5.Store the plasmid at -20 ℃. howley park industrial estateWebbWe have a modified pETM11 vector for the expression of SUMO3 fusion proteins available. This vector contains an optimized synthetic human sumo3 gene with appropiate restriction sites at the 3′-end of the sumo3 gene for the insertion of any gene of interest.. The small human SUMO3 protein (81 aa, 9.2 kDa) can be used as an N-terminal fusion partner for … howley park road east leeds ls27 0bnWebb中文名称： His和SUMO双标签质粒英文名称： pET-N-His-PreScission-SUMO 产品规格： 1μg 100μg 发货周期： 1～3天产品价格：询价本产品仅可用于科研实验，严禁用于其他 … howley park morleyWebb28 sep. 2024 · Natural source: Common Name: SARS-CoV-2 Taxonomy ID: 2697049 Superkingdom: Viruses Kingdom: not available Genus/species: Betacoronavirus HCoV … howley park ls27 0fu