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Hif 1a antibody

WebAnti-Hypoxia Inducible Factor 1 α Antibody, clone H1α67 clone H1alpha67, Chemicon®, from mouse; Synonyms: HIF-1 alpha, ARNT Interacting Protein, MOP1; find Sigma-Aldrich-MAB5382 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich WebHypoxia-inducible factor 1 (HIF1) is a heterodimeric transcription factor that plays a critical role in the cellular response to hypoxia (1). The HIF1 complex consists of two subunits, HIF-1α and HIF-1β, which are basic helix-loop-helix proteins of …

HIF1A Antibody (D5F3M) Cell Signaling Technology

WebHydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation … Web5 de jan. de 2024 · HIF1a Antibody (H1alpha 67) is a mouse monoclonal IgG 2b κ, cited in 183 publications, provided at 200 µg/ml. raised against amino acids 432-528 of HIF-1α of human origin. Anti-HIF1a Antibody (H1alpha 67) is recommended for detection of HIF-1α of mouse, rat and human origin by WB, IP, IF and IHC (P); also reactive with additional … highway 79 brewery https://qtproductsdirect.com

HIF-1 alpha antibody (20960-1-AP) Proteintech - ptglab

WebCytoplasmic in normoxia, nuclear translocation in response to hypoxia. Specificity. This antibody is directed against human HIF-1a hydroxyP564 and is specific for the … WebFigure 1. HIF-1a staining of HeLa cells. Anti-HIF-1a antibody [EPR16897] (ab179483) was used at 1/50 dilution with goat anti-rabbit IgG (Alexa Fluor ® 488) secondary antibody. Cells were treated with 0.6 nM CoCl 2 for 6 hours. Application. Recommended product. Western blot, immunocytochemistry. Anti-HIF-1a antibody (ab179483) ELISA. WebSummary. HIF1 alpha antibody recognizes HIF1 alpha (HIF1a) protein, which is a subunit of the heterodimeric transcription factor hypoxia-inducible factor-1 (HIF-1). HIF-1 is a … highway 77 seafood lincoln alabama

Anti-HIF1AN/FIH-1 antibody (ab187524) Abcam

Category:HIF-1alpha/HIF1A Mouse Monoclonal Antibody (HIF …

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Hif 1a antibody

HIF1A Monoclonal Antibody (mgc3), Invitrogen™

WebHIF1A Antibodies Antibodies that detect HIF1A can be used in several scientific applications, including Immunohistochemistry, Western Blot, Immunocytochemistry, Flow … WebHIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of …

Hif 1a antibody

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WebTo determine whether HIF-1α binds these HREs, we performed a CHIP assay using anti-HIF-1α antibodies in A549 cells cultured under hypoxic (1.5% O 2) or normoxic (21% O 2) conditions. DNA fragments containing the HRE1 and/or HRE2 binding sites were significantly more abundant under hypoxic conditions ( Figure 4G ), suggesting that HIF … WebHIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of …

WebDetection of Human HIF‑1 alpha /HIF1A by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 150 µM CoCl 2 for 16 hours. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat HIF-1a/HIF1A Antigen-affinity Purified Polyclonal Antibody, followed by HRP-conjugated Anti … WebRabbit polyclonal HIF-1 alpha antibody. Validated in WB and tested in Recombinant fragment. Cited in 46 publication(s). Independently reviewed in 1 review(s). Immunogen …

Web11 de abr. de 2024 · HIF-1α activates TMEM237 transcription in hypoxic HCC cells. We previously investigated hypoxia-induced transcripts in Hep3B cells using a microarray assay [].The microarray data (GSE155505) showed that TMEM237 was significantly upregulated in Hep3B cells under hypoxic conditions (Fig. 1a). Then, RT‒qPCR and Western blotting … WebI've had good luck with HIF-1a antibody Cat#3716 from Cell Signaling Tech. As previously suggested hypoxic chamber helps, however, if you do it quickly you don't need the chamber.

Web11 de nov. de 2015 · We identified HIF-1α binding sites across the genome, with binding sites showing enrichment for an RCGTG motif, showing conservation with the mammalian hypoxia response element. Conclusions: Transcriptome analysis of vhl mutant embryos detected activation of key hypoxia response genes seen in human cell models of …

WebHIF-1 alpha Antibody Immunofluorescence validation (1:100 dilution) in Cholangiocyte organoids (Cat no:20960-1-AP) FH. Christian (Verified Customer) (01-07-2024) Unfortunately much background an many unspecific bands in lysates of human chondrocytes and BV2 microclia cells (less unspecific bands in rat chondrocytes). highway 79 buckleWebHIF-1 alpha Antibody Immunofluorescence validation (1:100 dilution) in Cholangiocyte organoids (Cat no:20960-1-AP) FH. Christian (Verified Customer) (01-07-2024) … small spotlights for shelvesWebHIF-1 or hypoxia inducible factor 1 (predicted molecular weight 93kDa), is a transcription factor commonly referred to as a "master regulator of the hypoxic response" for its … highway 78 ridgeville scWeb8 de set. de 2024 · Flamant L, Notte A, Ninane N, Raes M, Michiels C. Anti-apoptotic role of HIF-1 and AP-1 in paclitaxel exposed breast cancer cells under hypoxia. Mol Cancer. 2010;9:191 pubmed publisher Ameri K, Luong R, Zhang H, Powell A, Montgomery K, Espinosa I, et al . small spotlights outdoorWebDescription: This ESEE122 monoclonal antibody reacts with hypoxia inducible factor-1 alpha. The hypoxia inducible factor (HIF-1) is a transcription factor composed of an inducible alpha form and the … small spotlightshttp://www.abways.com/showproduct.asp?cid=CY5197 small spot rocking recliner chairWebDescription: This Mgc3 monoclonal antibody detects hypoxia-inducible factor 1 alpha (HIF-1 alpha) from human, non-human primate, bovine, mouse and porcine cells. This antibody does not cross-react with ARNT … small spotlight bulb screw in