Cyto-id® green detection reagent
WebIn brief, cells were washed with PCR PBS (supplemented with 5% FBS) and then Gene Primer sequence mixed with Cyto-ID Dual Detection Reagent solution (Cyto-ID Green Detection Reagent and GAPDH Hoechst 33342). WebOct 20, 2016 · The Cyto-ID ® Green Reagent is a cationic amphiphilic tracer dye which selectively labels pre-autophagosomes, autophagosomes, and autophagolysosomes. …
Cyto-id® green detection reagent
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WebDec 22, 2024 · The cell residues were washed twice with the assay buffer, and 100 μl of Dual Detection Reagent (2 μl of Cyto-ID Green Detection Reagent and 1 μl of Hoechst 33,342 Nuclear Stain in 1 mL of cell culture medium) was then dispensed in each well. The cells were protected from light and incubated at 37°C for 30 min. WebFeb 1, 2024 · After 24 h of laser irradiation, cells were labeled with CYTO-ID® Green. All the fluorescent images were captured using Cytation5 cell imaging multi-mode reader with objective lens 20×, followed by uniform processing using Cytation5 software (bar = 100 μm). ... To assess autophagy, we used CYTO-ID® Autophagy Detection Kit 2.0 based on a …
WebJan 16, 2016 · Cyto-ID Green concentration contains 1 μl of Cyto-ID Green Detection Reagent in 1 ml cell culture medium. It was then mixed well and incubated for 30 min under standard tissue culture conditions at 37 °C, 5 % CO 2 in the dark. At the end of staining procedure, the Cyto-ID containing medium was washed with PBS. WebCellEvent™ Caspase-3/7 Green Detection Reagent is a cell-permeant reagent that consists of a four-amino acid peptide (DEVD) conjugated to a nucleic acid-binding dye. …
WebAug 6, 2024 · Autophagy analysis was performed by incubating cells with bafilomycin A1 for 30 min at 37 °C prior to treatment with CYTO-ID Green Detection Reagent, and then analyzing fluorescence by flow cytometry using the … WebDec 1, 2012 · The Cyto-ID green reagent (Cyto-ID) is a. ... Lyso-ID Red Detection Reagent (Lyso-ID) were from Enzo. Life Sciences. Cell culture. Bovine aortic endothelial cells (BAEC) were obtained from.
WebThe CYTO-ID ® Autophagy detection kit provides a rapid, specific and quantitative approach for monitoring autophagy in live cells by …
Web• Here, Cyto-ID Green was validated using fluorescence microscopy to demonstrate the colocalization of RFP-LC3 and Cyto-ID Green in a starvation model using HeLa cells. • … inception stairsWebMay 4, 2024 · The pattern of vacuole labeling 4 h after ER photodamage in the presence vs. absence of 5 μM cycloheximide with ERTracker Green, a fluorescent probe for the ER, is … inception stateWebOct 1, 2024 · The autophagy level of haemocytes was detected with CYTO-ID® Autophagy Detection Kit (Enzo, Switzerland) according to the instruction. ... for 3 h, and then washed two times with M-L15 medium. The haemocytes on the slide were incubated with 200 μL CYTO-ID® Green Detection Reagent (diluted 1:500 in M-L15 medium) at 37 °C in dark … inception stageWebThe DYNEX Technologies Automated ELISA System is a cutting edge computer-controlled processing system that achieves full automation of ELISA assays and surrounding … income tax act 1952WebIncase of Low CYTO-ID Green dye staining in all treatments, increase the reagent concentration (500X dilution of the dye is recommended) and the incubation time. ... grow the cells on coverslips after respective treatment incubate them with the dual detection reagent (Cyto‐ID dye and Hoechst 3342 nuclear stain) at 37 ºC for 30 minutes in the ... income tax act 194nWebAug 18, 2015 · TMRE (A), MitoSox Red (B), Cyto-ID (C), and Mitotracker green (D) ... (Invitrogen) for 15 min at 37°C to measure mitochondria-associated ROS, or 1:400 Cyto-ID autophagy detection reagent (Enzo Life Sciences) for 30 min at 37°C to measure autophagosomes. Flow cytometry and cell sorting were performed on the LSR II and Aria … inception steamWebCyto-ID Green autophagy dye staining procedure for autophagy detection Cyto-ID Green solution was prepared by mixing the dye with 1× assay buffer. The cell sample was centrifuged, and the dye was pipetted in for image-based or flow cytometry, respectively. The sample was shielded from direct light and incubated for 30 min at 37°C, income tax act 1947